Artificial light at night, that is mainly generated by urban areas and roads. Light is scattered and reflected back to the ground far away from urban centers thus effectively exposing large areas. By modifying the photoperiod, this phenomenon alter physiology, behaviour and activity, and interactions between organisms. Owing to its multifarious effect, artificial light at night is now recognized has a global potential hazard for human health, wildlife, and ecosystems. However, we still largely lack biological data to assess the effects in natural populations and spatial model of theses effects. This project investigate these issues and focuses on amphibians as model organisms by combinining in situ measurements, controlled experiments in the lab, and GIS modelling.